Feasibility for Urine Modified Nucleosides as a Tumor Mark in Lung ...

[Abstract]

Lung cancer is a most common carcinoma of human being. Its incidence has been increasing quickly in China recently. Most of patients with lung cancer have reached advanced stage while diagnosed owing to lack of an effective method in finding it in early stage. The general survey is an effective method of finding lung cancer in early stage. The tumor markers in body fluids will help to increase the rate of selectivity in survey. However, most of serum tumor markers as the protein of cell differentiation have narrow detection scope and low sensitivity as for lung cancer with complex differentiation. The genetic tumor markers also could not be well applied for the survey because of high individual difference of patients and low stability of the method.Modified nucleosides (MN) as main catabolites of tRNA can not be reutilized and excreted in urine finally. The levels of urine modified nucleosides (UMN) could reflect the turnover rate of tRNA. Because the malignant cells have rapid metabolic rate of tRNA, the cancer patients will excrete more MN to their urine than the healthy people. Therefore, the UMN might become a universal biochemical marker of lung cancer survey and diagnosisIt was reported that the MN as a valuable tumor marker had been used in diagnosing different cancers, such as leukemia, lymphoma, breast cancer, et al. But few reports were found about UMN as a tumor marker applied in diagnosing lung cancer.Object: To analyze whether the UMN could be used as a tumor marker in diagnosing lung cancer, and evaluating therapy effect and monitoring prognosis of lung cancer patients.Method: The levels of 15 UMN, including pseudouridine (Pseu), adenosone (A), uridine (U), cytidine (C), 5-methyluridine (m5U), inosioe (I), 1-methylinisine(m1I), N4-acetylcytidine (ac4C), guanosine (G), xanthosine (X), 2-methylguanosine (m2G), N6-methyladenosine (m6A), 1-methyladenosine (m1A), N2,2-methylguanosine (m22G) and 1-methylguanosine (m1G) were determined by high-performance liquid chromatography (HPLC) in 50 patients with lung cancer, 20 patients with pulmonary infectious diseases and 50 healthy persons. The serum carcinoembryonic antigen (CEA) levels of 15 patients with lung cancer were detected by radioimmunoassay (RIA). Result:1. The levels of 15 UMN in patients with lung cancer were significantly higher than those in healthy persons (p<0.001). The levels of 14 UMN in patients with pulmonary infectious diseases were significantly higher than those in healthy persons (p<0.05) except m5U. The levels of 11 UMN in patients with lung cancer were significantly higher than those in patients with pulmonary infectious diseases except C, U, I and A. Within the 11 UMN, the increasing levels of X and m5U were significant especially (p<0.001).2. The levels of 15 UMN in patients with lung cancer after effective chemotherapy were significantly lower than those before treatment (p<0.001).3. The levels of 15 UMN in different pathologic patterns, including squamous cell carcinoma, adenocarcinoma, large cell carcinoma and small cell carcinoma were not significantly different (p>0.05).4. The levels of 15 UMN had significant positive correlation to the stage I ? II and stageIII, stageIV (p<0.001). There had no correlation to stageIII and stageIV (p>0.05).5. The positive detective rates of 13 UMN in lung cancer of patients varying from 40% to 80% were higher than that of serum CEA being 33%, except for C (7%) and A (20%).Conclusion:1.The levels of 15 UMN in patients with lung cancer were significantly higher than those in healthy persons.2.The levels of 15 UMN in patients with lung cancer changed considerably paralleling with the changes in the clinical responses during chemotherapy. So it may be applied to monitoring prognosis and selecting sensitive chemotherapy drugs in lung cancer therapy.3. The levels of 15 UMN in lung cancer patients with different pathologicpatterns were no significant difference, but have significant positive correlation to the clinical stages. As a result,

Title: Feasibility for Urine Modified Nucleosides as a Tumor Mark in Lung Cancer Survey

Category: Cervix Cancer

Filename: Feasibility for Urine Modified Nucleosides as a Tumor Mark in Lung Cancer Survey.pdf

Pages: 164

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